This column will review the preparation of standard solutions by simple and serial dilution using standard pipettes and flasks. Only the uncertainties in the volumetric glassware, however, will be considered to answer the question: “Is my dilution accurate enough?” It will be assumed that all the other factors. Direct dilution vs serial dilution method. The AZ researchers generated their range of compound concentrations using two different techniques. In the first instance, they performed serial dilutions with 5% DMSO as the diluent. They also generated a concentration gradient with a direct dilution using the.

17.2.4.3 Sample Dilution Serial dilutions of samples are made using EMEM10 as the diluent in 8-well Lab-Tek slides. Typical dilutions are 1:2.5, 12.5, 62.5, and 312.5 in a final volume of 0.1 mL (a screening test can be performed by making two dilutions, e. Download Tuneup Utilities 2012 Portable. g. 1:5 and 1:25).

Alternatively, the dilutions can be prepared in 96-well plates and 0.1 mL transferred to the Lab-Tek slides. These samples are then further diluted by adding 0.1 mL of the challenge dose of RABV yielding final dilutions of 1:5, 1:25, 1:125, and 1:625 (1:10 and 1:50 for screening dilutions) (see Figure 17.3 for picture of slide). 3.1 Serial dilution Serial dilution generally refers to selection preformed in the standard growth regimes typically used in the lab: flasks, test tubes, solid media, or 96-well plates. El Amante Perfecto Pdf Viewer here. Cultures are usually allowed to grow through a normal growth curve, with daily transfer of a small volume of the expanded culture into fresh medium. Serial dilution has many advantages: the materials necessary are typically already present in the lab and require no special engineering.